2017-8-11 16:0:32025-04-30 05:43:55

title

yhdP

mpfA

_ec

description

potential magnesium efflux pump, mutation suppresses defects in 70 S ribosome formation

magnesium efflux pump, mutation suppresses defects in 70 S ribosome formation

locus

BSU09550

BSU_09550

geneLength

1332

1335

product

potential magnesium efflux pump

magnesium efflux pump

names

yhdP

mpfA

yhdP

outlinks

bsu

BSU09550

BSU_09550

The protein

Protein family

UPF0053 family (according to Swiss-Prot)

[SW|UPF0053 family](according to UniProt)

The protein

Paralogous protein(s)

[[protein|YhdT]], [[protein|YrkA]], [[protein|YqhB]], [[protein|YugS]]

[[this]]

The protein

[SW|Domains]

[SW|DUF21 domain] (5-201)

2 [SW|CBS domain]s (220-282, 284-344)

[SW|CNNM transmembrane domain] (aa 1-201) (according to UniProt)

[SW|DUF21 domain] (aa 5-201)

2 [SW|CBS domain]s (aa 220-282, aa 284-344) (according to UniProt)

The protein

[SW|Localization]

cell membrane (according to Swiss-Prot)

cell membrane (according to UniProt)

Biological materials

Mutant

MGNA-A696 (yhdP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/696 NBRP B. subtilis, Japan]

HB24928 (Δ[[gene|mpfA]]), available in [SW|John Helmann]'s and [SW|Jörg Stülke]'s labs

MGNA-A696 (yhdP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/696 NBRP B. subtilis, Japan]

BKE09550 (Δ[[gene|mpfA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGT

BKK09550 (Δ[[gene|mpfA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGT

Biological materials

Expression vector

pGP2931 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

10960106, 22383849, 25182490, 27933050

10960106, 22383849, 25182490, 27933050, 29967120, 31415562, 31864811, 31964700

Gene

Phenotypes of a mutant

suppression of the growth defects of [[gene|rplA]] and [[gene|rplW]] mutants [pubmed|29967120]

partial suppression of sporulation frequency of a [[gene|rplA]] mutant [pubmed|29967120]

suppresses the Mn²⁺ sensitivity of a [[gene|mntR]] [[gene|mntH]] double mutant [pubmed|31964700]

increased tolerance to Mn²⁺ and Co²⁺ [pubmed|31964700]

resistant to high Mg²⁺ concentrations [pubmed|31964700]

Biological materials

Expression vectors

pGP2931 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab